Autoimmune telegony txoj kev xav

2024 Tus sau: Seth Attwood | [email protected]. Kawg hloov kho: 2023-12-16 16:06

Kev coj ncaj ncees, raws li kev tiv thaiv pej xeem kev coj ua, tiv thaiv cov txheej txheem degeneration ntawm hom.

Telegony yog ib qho kev ruaj khov sawv cev ntawm qee tus neeg yug tsiaj, raws li kev soj ntsuam ntawm qhov txawv txav qhov tseeb ntawm kev hloov pauv hauv cov tsos ntawm cov xeeb leej xeeb ntxwv, nrog rau kev tsis sib haum xeeb.

Raws li cov ntsiab lus niaj hnub no, feem ntau ntawm qhov tseeb "ua kom pom qhov tshwm sim ntawm telegony" yog qhov tshwm sim ntawm cov xeeb ntxwv ntawm cov cim uas tsis nyob hauv lawv cov niam txiv tam sim ntawd, tab sis qhov ntawd muaj nyob hauv cov poj koob yawm txwv nyob deb. Ib qho piv txwv ntawm phau ntawv kawm yog kev txheeb xyuas qhov zais (recessive) zoo li qhov tshwm sim ntawm kev sib cais ntawm qee qhov kev sib txuas ntawm niam txiv genotypes, nrog rau atavisms, kev hloov pauv hloov pauv hloov pauv hloov pauv uas rov kho cov ntaub ntawv caj ces hloov pauv los ntawm kev hloov pauv tseem ceeb (xws li cov tsos ntawm tus Tsov tus tw hauv tus txiv neej).

Dab tsi yog qhov tshwm sim rau cov kev hloov pauv thib ob? Kab lus no qhia txog qhov cuam tshuam ntawm cov tshuaj tiv thaiv kab mob hauv cov genome raws li qhov ua rau mutagenic.

Lub autoimmune txoj kev xav ntawm telegony qhia tau hais tias cov kev pom tshwm sim los ntawm kev cuam tshuam ntawm cov khoom siv caj ces, los ntawm cov tshuaj tiv thaiv kab mob, uas tau tsim nyob rau hauv ntau yam kab mob, sodomy (qhov quav poj niam, ntau tus neeg koom tes, ntau zaus ntawm kev sib raug zoo), thiab. raws li nyob rau hauv homosexuals thiab cov neeg raug tsim txom ntawm pedophiles … Ntxiv mus, qhov kev nplua nuj ntawm kev sib deev, qhov hnyav dua thiab ntau yam kev tiv thaiv kab mob tiv thaiv kab mob ntawm cov me nyuam raug tsim thiab ntau DNA cuam tshuam rau cov xeeb ntxwv. Feem ntau ntawm cov cev xeeb tub no tau txiav tawm, vim qhov deformity uas tsis sib haum nrog lub neej. Feem ntau, cov me nyuam tuag los ntawm kev mob qog noj ntshav, los yog yug los nrog kev txawv txav ntawm lub cev, thiab feem ntau ob niam txiv ua tsis muaj menyuam.

Social tseem ceeb ntawm qhov tshwm sim

Hauv peb lub cev muaj ntau cov kab mob, streptococci, staphylococci, thiab lwm yam, tab sis lawv tsis tua peb ntev npaum li kev tiv thaiv kab mob. Thiab thaum peb tuag, cov kab mob no sai sai decompose lub cev.

Yog li ntawd yog social. Nws muaj txog li 1% ntawm cov neeg nyiam sib deev (tiag tiag, thiab tsis hloov kev taw qhia raws li kev cuam tshuam ntawm lub xeev. Kev tshaj tawm ntawm Western lub teb chaws, thiab sectarians). Lub koom haum, nyob rau theem sib txawv ntawm nws txoj kev loj hlob, castrates lawv, pob zeb rau lawv, los yog dai lawv, kho lawv los yog kaw lawv, uas tsis pab rau lawv kev loj hlob. Qhov ntawd yog, haiv neeg ib txwm tsis kam ua siab ntev thiab muaj kev tiv thaiv. Thaum kev coj ncaj ncees hauv zej zog poob (txawm nws tus kheej los yog nyob rau hauv lub meej mom ntawm cov neeg tseem ceeb), cov neeg nyiam sib deev, zoo li cov kab mob no los yog pwm, kis, hloov lawv lub tswv yim Sodom rau hauv lub siab ntawm cov neeg nyob ib puag ncig lawv, ua kev nyiam sib deev hauv lub xeev. ideology, pib los ntawm tsev kawm ntawv, thiab yog li rhuav tshem cov pej xeem nyob rau hauv ntau txoj kev: homosexuals lawv tus kheej tsis yug, cov neeg nyob rau hauv sodomy tau txais autoimmune infertility, lwm yam kev sib deev kab mob, thiab txawm tias lawv yug los, lawv yug los degenerates uas yog txawm. muaj feem ntau rau kev coj tsis ncaj ncees thiab kev sib deev sib txawv. Hauv Tebchaws Meskas, lawv tau tso ntshav rau cov neeg nyiam sib deev, thiab lawv tsis sim nws rau cov tshuaj tiv thaiv phev, thiab pom meej tias lawv tsis nug peb. Tag nrho cov no, dhau ntau tiam neeg, ua rau cov neeg ploj mus.

Cov neeg uas muaj sia nyob hauv keeb kwm yog khaws cia los ntawm kev coj ncaj ncees. Tsuas yog tus poj niam sib deev hauv tus poj niam uas xeeb tub yam tsis muaj kev sib deev perversion guarantees qib qis ntawm autoimmune mutations, thiab kev txuag ntawm heredity.

Txawm li cas los xij tus neeg tsim nyog tau txais kev hloov pauv uas ua rau nws tsim nyog: evolution, divine creation los yog cov noob caj noob ces ntawm ib tug ntau tsim lub siab, sodomy, thiab loj heev kis ntawm pederasty, pedophilia, muaj peev xwm rov qab tau cov noob pool ntawm tib neeg mus rau nws thawj lub xeev..

Kev txhawb nqa ntawm tib-poj niam txiv neej kev sib raug zoo, sodomy, dhau los ua tsoomfwv Meskas txoj cai. Hauv Tebchaws Europe, lawv tab tom sim ua txhaum cai pedophilia. Qhov no yog txoj cai ntawm kev tua neeg ntawm cov neeg hauv ntiaj teb, uas tau cog lus, qee zaum los ntawm kev quab yuam, kev ywj pheej Western qhov tseem ceeb, nrog rau lub hom phiaj ntawm kev ua kom degradation thiab lub cev tshem tawm cov pej xeem uas ntau dhau, los ntawm kev xav ntawm cov neeg tseem ceeb.

Qhov xav tau kev coj ncaj ncees los khaws cov pej xeem tau lees paub los ntawm kev tshawb fawb:

Pov thawj:

Ntau nplooj ntawv hauv Is Taws Nem nrog cov ntaub ntawv hais txog ASA ntawm cov neeg sodomites thiab homosexuals, tom qab txuas rau lawv, ploj ntawm lub network. Nov yog cov ntawv.

Ivan Kurennoy

Antisperm antibodies (ASA)los yog cov tshuaj tiv thaiv kab mob rau cov phev antigens yog immunoglobulins uas tsim los ntawm lub cev tiv thaiv kab mob ntawm cov poj niam thiab cov txiv neej uas txwv cov phev ua haujlwm. Antisperm antibodies yog ib qho ntawm cov ua rau muaj kev tiv thaiv kab mob.

Nyob rau hauv lub cev noj qab haus huv ntawm cov poj niam thiab cov txiv neej, cov tshuaj tiv thaiv tiv thaiv phev antigens tsis tsim.

Hauv cov txiv neej, lawv cov tsos mob yog txuam nrog kev ua txhaum ntawm kev ncaj ncees ntawm cov ntshav-testicular barrier. Nws yog ib tug kab mob lom uas cais cov seminiferous tubules thiab cov hlab ntsha. Nws qhov kev puas tsuaj tshwm sim nrog kev raug mob rau cov noob qes, kab mob thiab kab mob ntawm cov kab mob gonads (epididymitis, orchitis), qog nqaij hlav qog noj ntshav, nrog cryptorchidism, varicocele, tom qab kev phais mob ntawm cov qog ntshav. Tom qab kev phais mob rau cryptorchidism (cov noob qes tsis tau nkag mus rau hauv lub scrotum), cov tshuaj tiv thaiv kab mob tsis tau kuaj pom hauv cov menyuam yaus, thiab hauv cov txiv neej laus lawv tshwm sim hauv 40% ntawm cov neeg mob. ASA yog ib qho kev tshawb pom ntawm cov poj niam nyiam txiv neej thiab cov txiv neej muaj HIV.

Hauv cov poj niam, cov tshuaj tiv thaiv kab mob tshwm sim nrog cov tshuaj tiv thaiv autoimmune, kab mob. Lawv tuaj yeem tsim thaum qhov chaw mos mucosa puas los ntawm cov tshuaj tiv thaiv kab mob; yog tias cov phev nkag mus rau hauv lub plab zom mov thaum lub qhov ncauj lossis qhov quav; thaum cov phev nkag mus rau hauv lub plab kab noj hniav vim cov qauv ntawm qhov chaw mos; Nrog rau cov ntsiab lus siab ntawm leukocytes hauv ejaculate, cov spermatozoa ingress mus rau hauv qhov chaw mos, uas yog txuam nrog cov tshuaj tiv thaiv kab mob (kev sib txuas nrog bisexuals, los yog passive homosexuals). Ntxiv mus, qhov sib deev ntau dua muaj, qhov kev tiv thaiv kab mob ntau dua yog tsim.

Cov tsos mob ntawm cov tshuaj tiv thaiv kab mob ua rau muaj kev cuam tshuam ntawm cov txheej txheem fertilization, cuam tshuam nrog kev loj hlob ntawm tus me nyuam hauv plab.

Mechanisms ntawm cov nyhuv ntawm cov tshuaj tua kab mob antisperm ntawm kev ua me nyuam:

• txo cov phev motility,
• phev agglutination (gluing),
• thaiv kev nkag mus ntawm cov phev los ntawm cov hnoos qeev nyob rau hauv lub ncauj tsev menyuam, lawv kev nce qib los ntawm lub tsev menyuam thiab cov hlab ntsha fallopian,
• thaiv cov receptors ntawm cov phev taub hau, uas khi rau zona pellucida,
• kev ua txhaum ntawm capacitation (tshem tawm ntawm glycoprotein daim nyias nyias los ntawm cov phev cell, yam tsis tau npaj rau fertilization),
• kev tawm tsam ntawm acrosomal cov tshuaj tiv thaiv (kev hloov pauv biochemical ntawm lub taub hau),
• thaiv kev sib xyaw ntawm cov phev nrog oolemma (qe membrane),
• ua txhaum ntawm gamete fusion,
• inhibition ntawm embryo loj hlob,
• fragmentation ntawm DNA,
• ib qho kev cuam tshuam rau kev txuas ntawm lub embryo rau phab ntsa ntawm lub tsev menyuam.

Antisperm antibodies tsis yog ib txwm nrog los ntawm infertility, txawm li cas los xij, yog tias lawv muaj nyob hauv cov ntshav ntawm ib tus txij nkawm, kev xeeb tub tsis tshwm sim hauv 4 qhov ntawm 10. Yog tias tsis pom lwm qhov ua rau muaj menyuam tsis taus, cov tshuaj antisperm yog suav tias yog qhov ua rau..

Kev tsim cov tshuaj tiv thaiv rau cov phev thaum siv hnab looj tes

Puas siv hnab looj tes pab tshem tawm cov phev tshuaj tiv thaiv?

Tsis muaj. Thaum cov tshuaj tiv thaiv kab mob yog induced, lub cim xeeb hlwb raug tsim tawm sai sai ua cov tshuaj tiv thaiv kab mob tiv thaiv kev kis tus kab mob tom ntej. Qhov no yog lub hauv paus ntsiab lus ntawm kev txhaj tshuaj.

Hauv cov poj niam uas muaj cov tshuaj tiv thaiv kab mob phev, qhov tsis muaj kev sib cuag nrog cov phev vim yog siv cov hnab looj tes thaum sib deev yuav tsis cuam tshuam rau cov hlwb. Tom qab raug cov phev hlwb yuav sai sai ua rau zus tau tej cov anti-sperm antibodies dua.

S. S. Bitkin

Antibodies rau phev thaum lub sij hawm sib deev ntawm qhov quav

Cov tshuaj tua kab mob puas tuaj yeem tshwm sim los ntawm qhov quav?

Nyob rau hauv cov txiv neej - passive homosexuals, qhov zaus ntawm carriage ntawm antisperm antibodies yog siab heev. Ntxiv mus, yog tias lawv xav xeeb tub lawv tus kheej thiab cov teeb meem ntawm qhov muaj txiaj ntsig zoo tshwm sim, kev kuaj tshuaj tiv thaiv kab mob tau sau tseg.

Nco ntsoov tias hauv qhov kev sim, qhov tsos ntawm cov tshuaj tiv thaiv kab mob hauv cov tsiaj sim yog tshwm sim los ntawm qhov quav insemination.

Yog li, nws zoo nkaus li tias qhov kev nkag ntawm cov phev mus rau hauv qhov quav yuav ua rau tsim cov tshuaj tiv thaiv phev.

S. S. Bitkin

Pathogenesis ntawm txo fertility nyob rau hauv autoimmune tshuaj tiv thaiv spermatozoa

Bozhedomov V. A., Nikolaeva M. A., Ushakova I. V., Sporish E. A., Rokhlikov I. M., Lipatova N. A., Sukhikh G. T.

Lub hom phiaj ntawm kev kawm

Qhia txog kev sib txuas ntawm cov lus teb autoimmune tiv thaiv cov phev, lawv cov yam ntxwv ua haujlwm thiab qhov tseeb fertility.

Khoom siv thiab cov txheej txheem. Kev kuaj mob thiab kuaj mob ntawm 425 tus txiv neej los ntawm cov niam txiv tsis muaj menyuam hnub nyoog 18-45 xyoo tau ua tiav; Cov txiv neej fertile, uas nws tus poj niam cev xeeb tub ntawm 8-16 lub lis piam, tsim pawg tswj hwm (n = 82). Kev soj ntsuam cov phev tau ua tiav raws li qhov yuav tsum tau ua ntawm WHO, siv computerized phev tsom xam (CASA). Kev txiav txim siab ntawm cov tshuaj tiv thaiv kab mob (ASAT) hauv cov phev - MAR thiab ntws cytofluorometry, hauv cov ntshav ntshav - ELISA. Spontaneous thiab ionophore A23187-induced acrosomal reaction (AR) - siv ob chav fluorescent staining ntawm spermatozoa siv fluorescein-isothiocyanate-labeled lectin P. sativum thiab tetramethylrhodamine-isothiocyanate-labeled lectin A. Kev ntsuam xyuas ntawm oxidative stress (OS) yog ua los ntawm txoj kev ntawm luminol-dependent chemiluminescence. Chromosome kev puas tsuaj raug soj ntsuam los ntawm DNA fragmentation los ntawm chromatin dispersion nyob rau hauv ib tug inert agarose gel nrog kev ntsuam xyuas pom nyob rau hauv ib tug microscope ntawm halo tsim tom qab acid denaturation ntawm DNA thiab lysis ntawm nuclear proteins.

Cov txiaj ntsig tshawb fawb

Qhov poob ntawm qhov tseeb fertility yog proportional rau feem pua ntawm MAR-zoo phev. Cov tshuaj tiv thaiv autoimmune tawm tsam spermatozoa yog nrog los ntawm kev tsim cov pa oxygen ntau dhau. Muaj kev sib raug zoo ntawm cov txiaj ntsig ntawm MAR xeem thiab kev taug qab ceev ntawm cov phev, qhov amplitude ntawm lub taub hau oscillation, feem pua ntawm cov phev nrog ntxov ntxov thiab tsis tuaj AR, feem pua ntawm cov phev nrog DNA fragmentation thiab cov degree ntawm xws fragmentation.

Xaus

Cov yam tseem ceeb ntawm kev txo qis fertility hauv cov txiv neej nrog ACAT yog cov phev ua haujlwm tsis zoo: hyperactivation ntxov ntxov, nce thiab / lossis tsis tuaj AR, thiab nce DNA fragmentation. Lub pathogenesis ntawm pathospermia nyob rau hauv lub cev tsis muaj zog yog txuam nrog OS.

Ib qho ntawm cov ua rau txiv neej infertility yog autoimmune tshuaj tiv thaiv spermatozoa, uas yog nrog los ntawm zus tau tej cov antisperm antibodies - ASAT [1]. Nyob rau hauv lub xub ntiag ntawm ASAT, agglutination thiab ib tug txo nyob rau hauv phev motility tshwm sim, nkag mus rau hauv lub ncauj tsev menyuam mucus thiab fertilization ntawm lub qe yog impaired; muaj pov thawj tias ACAT tuaj yeem cuam tshuam tsis zoo rau kev loj hlob ntawm embryo thaum ntxov, implantation, thiab cev xeeb tub [2–7]. Txawm li cas los xij, pathogenesis ntawm txo qis fertility thiab nchuav menyuam nyob rau hauv lub xub ntiag ntawm ASAT yog tseem tsis meej.

Lub hom phiaj ntawm txoj kev tshawb no: los qhia txog kev sib raug zoo ntawm cov tshuaj tiv thaiv autoimmune tiv thaiv spermatozoa, lawv cov yam ntxwv ua haujlwm thiab kev muaj tiag tiag.

Cov ntaub ntawv thiab cov txheej txheem tshawb fawb

Kev kuaj mob thiab kuaj mob ntawm 425 tus txiv neej los ntawm cov niam txiv tsis muaj menyuam hnub nyoog 18-45 xyoo tau ua tiav; Cov txiv neej fertile, uas nws tus poj niam cev xeeb tub ntawm 8-16 lub lis piam, tsim pawg tswj hwm (n = 82).

Kev kuaj phev tau ua raws li cov cai ntawm WHO [8]. Cov phev zoo Performance index (ICS) tau xam - tus naj npawb ntawm cov phev spermatozoa nrog ib txwm morphology thiab nce motility hauv ejaculate (mln / ejaculate). Tsis tas li ntawd, cov phev motility tau soj ntsuam siv lub computer phev analyzer "MTG" (Medical Technology Vertriebs Gmbh, Lub teb chaws Yelemees), qhov kev pab cuam "medeaLAB CASA": curvilinear (khiav) tshaj tawm (VCL, μm / sec), rectilinear tshaj tawm (VSL, μm). / sec), amplitude ntawm kab rov tav txav ntawm lub taub hau (ALH, μm / sec) thiab linearity (LIN,%). Kev txiav txim siab ntawm ACAT IgG thiab IgA ntawm spermatozoa tau ua los ntawm MAR (mixed antiglobulin cov tshuaj tiv thaiv) txoj kev (Ferti Pro NV, Belgium) thiab los ntawm kev ntws cytometry (PCM) siv Facscan (Becton Dickinson, USA) thiab Bryte (Bio-Rad, Ltalis); hauv cov ntshav ntshav - siv Spermatozoa antibody ELISA (IBL, Lub Tebchaws Yelemees). Raws li cov txiaj ntsig ntawm MAR xeem, ib pawg ntawm cov neeg mob uas muaj kev tiv thaiv kab mob me me (MAR% IgG = 10–49%) thiab ib pab pawg nrog WHO autoimmune infertility (MAR% IgG> 50%) tau txheeb xyuas.

Txhawm rau ntsuas qhov tshwm sim ntawm tus kheej thiab ionophore-induced A23187 acrosomal cov tshuaj tiv thaiv (AR), peb siv txoj kev ntawm ob chav fluorescent staining ntawm spermatozoa siv fluorescein-isothiocyanate-labeled lectin P. sativum (Sigma, USA) thiab tetramethylrhodocamine-labeledyanateislabel., USA 9]. Kev ntsuam xyuas ntawm oxidative stress (OS) tau ua los ntawm kev txiav txim siab qhov kev siv ntawm cov dawb radical txheej txheem los ntawm luminol-dependent chemiluminescence [10] siv lub LKB-Wallac 1256 luminometer (Finland) thiab Chemiluminometer-003 (Russia). Qhov kev siv ntawm chemiluminescence tau txiav txim los ntawm lub teeb sum thiab qhov siab tshaj plaws luminescence amplitude, uas sib raug rau tus nqi ntawm kev tsim cov pa oxygen reactive (ROS). Kev puas tsuaj rau phev chromosomes yog tus cwj pwm los ntawm DNA fragmentation, soj ntsuam los ntawm chromatin dispersion (SCD-test, Spain) nyob rau hauv ib qho inert agarose gel nrog kev ntsuam xyuas pom nyob rau hauv ib lub microscope ntawm halo tsim tom qab acid denaturation ntawm DNA thiab lysis ntawm nuclear proteins [11]. Qhov feem pua ntawm cov spermatozoa nrog cov tsos mob ntawm apoptosis thiab qib ntawm halo tsim cuam tshuam tau raug soj ntsuam ntawm 5-point scale.

Kev txheeb xyuas cov ntaub ntawv tau ua tiav siv Statisica software pob (StatSoft, USA); qhov nruab nrab, M, S raug xam, qhov tseem ceeb ntawm qhov sib txawv tau raug ntsuas los ntawm Kev Ntsuas Cov Tub Ntxhais Kawm, Mann-Whitney thiab Fisher rau cov qauv ywj pheej, Chi-square, kev txheeb xyuas kev sib raug zoo (R, Gamma coefficients raug xam).

Cov txiaj ntsig tshawb fawb thiab kev sib tham

Hauv cov pab pawg ntawm cov neeg mob nrog ACAT, spermogram indices tau loj dua li cov txiv neej fertile (p <0.05–0.01), tab sis feem ntau cuam tshuam rau normozoospermia [8] thiab tsis txawv ntawm qhov sib txawv ntawm qhov ntsuas MAR (saib tab; p> 0.05).

Kev sib raug zoo ntawm qhov muaj ASAT thiab tus kheej spermogram tsis muaj zog. Muaj qhov sib txawv thaum siv ntau txoj hauv kev los txiav txim siab ACAT: IR tsis nyob ntawm MAR% IgG thiab IgA (p> 0.05), tus nqi ntawm ACAT hauv cov ntshav, raws li ELISA cov ntaub ntawv (p> 0.05), tab sis nws tau cuam tshuam tsis zoo. nrog rau feem pua ntawm gametes nyob, coated nrog ACAT IgG, raws li PCM (R = 0.29; p = 0.005). Qhov no lees paub cov ntaub ntawv tau los ntawm peb ua ntej [12].

Qhov ntsuas ntsuas - lub sijhawm ntawm kev tsis tuaj yeem muaj menyuam tsis taus (ABI), - ntawm qhov tsis sib xws, nyob ntawm qhov kev ntsuas MAR IgG txawm tias muaj normozoospermia (R = 0, 39; p = 0, 00001); Ntxiv mus, cov tshuaj tiv thaiv ntawm IgG chav kawm qhia txog 2 lub sij hawm muaj zog dua IgA (R = 0, 20; p = 0, 03); tsis muaj kev sib raug zoo ntawm DVB thiab PCM cov ntaub ntawv thiab cov ntshav ASAT cov ntsiab lus (ELISA) (p> 0.05).

Cov ntaub ntawv no qhia tau hais tias qhov txo qis ntawm kev muaj menyuam tawm tsam keeb kwm yav dhau los ntawm ASAT feem ntau yog los ntawm kev ua haujlwm tsis zoo ntawm cov phev spermatozoa thiab paub meej tias cov kev xav ntawm kev kuaj xyuas ASAT ntawm mobile gametes muaj feem cuam tshuam nrog kev muaj menyuam tiag tiag [8, 13].

Txhawm rau txheeb xyuas qhov muaj peev xwm fertilizing ntawm phev, qhov kev ntsuam xyuas ntawm cov phev capacitance (CS) thiab AR, uas muaj kev cuam tshuam, tab sis ob qhov xwm txheej sib cais, tau siv [14].

Kev soj ntsuam hauv computer ntawm kev txav mus los tau pom (Daim duab 1) tias nrog kev nce hauv qhov sib npaug ntawm ACAT-zoo spermatozoa, lawv cov rectilinear thiab curvilinear velocities, lub amplitude ntawm kab rov tav txav ntawm lub taub hau, piv txwv li.muaj cov cim qhia ntawm hyperactivation, uas yog suav hais tias yog ib qho kev tshwm sim ntawm Ks [15, 16].

Nquag, XC tshwm sim hauv cov poj niam cev xeeb tub nyob rau hauv qhov kev txiav txim ntawm cytokines, progesterone, thiab zona pellucida receptors thiab yog ib qho mob rau AR, txheej txheem tsim nyog rau qe nkag [16, 17]. XC ntxov ntxov ua ntej nkag mus rau poj niam lub cev tuaj yeem suav hais tias yog ib qho tseem ceeb uas txo qis fertility.

Yav dhau los, nws tau pom tias muaj kev sib raug zoo ntawm feem pua ntawm ACAT-positive spermatozoa thiab feem pua ntawm gametes uas ua rau poob lub acrosome [18, 19]. Raws li peb cov ntaub ntawv tshiab, hauv cov tshuaj tiv thaiv kab mob autoimmune tawm tsam spermatozoa, muaj ob hom kev mob: redundancy ntawm spontaneous thiab insufficiency ntawm induced AR. Raws li pom hauv Fig. 2, cov kev ua txhaum cai no feem ntau pom ua ke. Cov phev hlwb ntau dua yog them nrog ACAT, qhov pom tau zoo dua cov kab mob no yog: tsuas yog 40% ntawm cov txiv neej uas muaj lub cev tsis muaj zog tiv thaiv kab mob raws li WHO tswj hwm ib txwm muaj AR, uas yog qhov tsawg dua hauv kev xeeb tub (p <0, 001) thiab hauv pab pawg. nrog MAR% IgG = 10– 49% (p <0.01).

Cov lus nug tseem ceeb tseem tsis tau paub meej: cov tshuaj tiv thaiv autoimmune tiv thaiv spermatozoa lawv tus kheej ua rau AR mob, lossis ACAT cuam tshuam nrog gametes, qhov acrosome uas tsis zoo vim yog kev ua ntawm qee yam - caj ces lossis exogenous.

Cov ntaub ntawv tau txais los ntawm qhov tsis zoo ntawm cov tshuaj tiv thaiv autoimmune ntawm Kc thiab AR piav qhia cov txiaj ntsig ntawm kev tshawb fawb uas txo qis hauv kev ua tiav ntawm qe fertilization hauv vitro nrog phev nrog ACAT tau txais [5, 7]. Nyob rau tib lub sijhawm, tsis muaj qhov tseeb ntawm qhov teeb meem no, txij li, raws li cov kws sau ntawv ntawm kev tshuaj xyuas tsis ntev los no thiab kev tshuaj ntsuam xyuas meta [20], qhov muaj ASAT tsis cuam tshuam rau feem pua ntawm cev xeeb tub nrog IVF thiab hloov embryo.

Peb cov ntaub ntawv tso cai rau peb piav qhia txog qhov nce hauv feem pua ntawm kev xeeb tub tsis tau pom los ntawm qee tus kws sau ntawv hauv lub xub ntiag ntawm ASAT [2, 3].

Nws tau raug tsim los hais tias thaum lub sij hawm autoimmune cov tshuaj tiv thaiv spermatozoa, qhov kev faib ua feem ntawm gametes nrog kev cuam tshuam hauv cov qauv ntawm chromosomes nce. Qhov nruab nrab, qhov feem pua ntawm cov spermatozoa nrog DNA fragmentation hauv lub cev tsis muaj zog (MAR%> 50%) yog 1.6 npaug ntau dua hauv MAR% = 10–49% (p = 0, 003); nyob rau hauv qhov ib txwm muaj - 10 thiab 55% ntawm qhov tseem ceeb hauv cov neeg mob ntawm cov pab pawg no (p <0.01), feem. Rau cov degree ntawm DNA fragmentation, qhov sib txawv yog tsawg pronounced - 1, 25 zaug (p = 0.01); nyob rau hauv qhov ib txwm muaj - 21 thiab 55% ntawm cov qauv, feem. Kev sib raug zoo ntawm MAR IgG thiab DNA puas yog ncaj qha: R = 0.48 (p = 0.003) rau feem pua spermatozoa nrog DNA fragmentation thiab R = 0.43 (p = 0.007) rau qib ntawm chromatin dispersion (Fig. 3) …

Qhov tshwm sim ntawm qhov ua rau ntxov ntxov AR thiab nce DNA fragmentation nyob rau hauv autoimmune tshuaj tiv thaiv spermatozoa yog OS. Nws paub tias ntau dhau ntawm ROS nyob rau hauv cov txheej txheem sib kis thiab mob, varicocele, ntshav qab zib mellitus, thiab qee yam kab mob ua rau muaj kev puas tsuaj rau cov phev daim nyias nyias, txo qis hauv lawv txoj kev mus los thiab kev puas tsuaj ntawm fertilizing muaj peev xwm [21-23]. Nyob rau hauv cov ntaub ntawv no, ROS muaj peev xwm ncaj qha mus ua puas DNA ntawm chromosomes thiab pib apoptosis ntawm spermatozoa [23-25], raws li ib tug tshwm sim ntawm uas cev xeeb tub feem ntau xaus nyob rau hauv spontaneous rho menyuam [26], Congenital anomalies thiab mob qog noj ntshav thaum yau yuav tshwm sim [25, 27]. Lub peev xwm ntawm ACAT cuam tshuam txoj kev loj hlob ntawm lub embryo tau tham ntev lawm [4], muaj cov ntaub ntawv sim ua pov thawj qhov no [28]; Cov tshuaj tiv thaiv kab mob tiv thaiv cov phev hauv cov poj niam yog suav tias yog qhov tseem ceeb hauv kev cog qoob loo tsis zoo [29]. Tab sis nws tsis paub tias puas muaj kev puas tsuaj rau DNA qauv thaum lub sij hawm autoimmune tshuaj tiv thaiv cov phev. Peb yog thawj tus tsim [22, 30] tias nyob rau hauv cov neeg mob uas muaj lub cev tsis muaj zog, ROS ntau lawm hauv cov phev yog nce ntxiv: muaj kev sib raug zoo ntawm MAR% IgG thiab ROS ntau lawm (R = 0.34; p = 0.03); kev sib raug zoo ntawm ROS ntau lawm thiab tus nqi ntawm IgG ntawm gametes tseem muaj zog, raws li PCM cov ntaub ntawv (R = 0.81; p = 0.007).

Lub luag hauj lwm ntawm OS nyob rau hauv lub pathogenesis ntawm txiv neej lub cev tsis muaj zog yog paub tseeb hais tias los ntawm kev siv cov antioxidants uas muaj peev xwm chemically khi ntau tshaj ROS thiab tiv thaiv cell puas. Thaum kho, muaj qhov txo qis ntawm ACAT-zoo spermatozoa thiab normalization ntawm AR [31].

Cov ntaub ntawv tau qhia meej txog cov pathogenesis ntawm txo fertility nyob rau hauv autoimmune tshuaj tiv thaiv cov phev, thiab ua rau ib tug ntau ceev faj txog kev siv IVF txoj kev, incl. intracytoplasmic phev txhaj nyob rau hauv cov ntaub ntawv ntawm lub cev tsis muaj zog txiv neej infertility, thaum phev OS tshwm sim thiab DNA fragmentation yog nce. Kev tshawb fawb ntxiv nyob rau hauv cov lus qhia no yog pom zoo kom paub meej thiab ntau tus yam ntxwv ntawm kev sib raug zoo ntawm DNA fragmentation, ntawm ib sab, thiab cov txiaj ntsig ntawm IVF kev kho mob ntawm lub cev tsis muaj zog ntawm cov txiv neej, ntawm lwm tus.

Xaus

Txiv neej fertility nyob rau hauv autoimmune tshuaj tiv thaiv spermatozoa yog txo nyob rau hauv kev faib ua feem rau qhov feem ntawm mobile gametes them nrog ACAT, thiab yog tshwm sim los ntawm functional disorders ntawm spermatozoa: hyperactivation ntxov ntxov, AR disorders thiab nce DNA fragmentation txuam nrog OS.