Kev cuam tshuam ntawm ultrasound ntawm cov tsiaj thiab cov nroj tsuag

2024 Tus sau: Seth Attwood | [email protected]. Kawg hloov kho: 2023-12-16 16:06

Cavitation hauv ib puag ncig yog qhov laj thawj tseem ceeb rau kev puas tsuaj ntawm ultrasound ntawm cov kab mob. Yog hais tias qhov tsim ntawm npuas yog suppressed los ntawm kev ua kom lub siab sab nraud, ces qhov kev puas tsuaj ntawm protozoa txo. Qhov yuav luag tam sim ntawd tawg ntawm cov khoom hauv thaj chaw ultrasound yog tshwm sim los ntawm huab cua npuas lossis carbon dioxide nyob rau hauv cov nroj tsuag hlwb dai hauv cov kab mob no.

Qhov no qhia tau hais tias qhov sib txawv loj loj tshwm sim thaum lub sij hawm cavitation ua rau rupture ntawm cell membranes thiab tag nrho cov kab mob me. Cov nyhuv ntawm ultrasound ntawm ntau hom fungi tau kawm ntau zaus. Yog li, ultrasound tau ntse siv hauv phytopathology. Ntawm qab zib beet noob kab mob lawm nrog Phoma betae, Cercospora beticola, Alternaria sp. los yog Fusarium sp., nws muaj peev xwm ua kom puas cov fungi thiab cov kab mob no zoo dua los ntawm lub sij hawm luv luv irradiation nrog ultrasound hauv dej dua li nws tau ua nrog etching. Irradiation ntawm cov noob nrog ultrasound thaum hnav khaub ncaws tseem ceeb txhim kho cov nyhuv ntawm fungicidal los yog bactericidal tshuaj. Yog vim li cas, thaj, yog lub suab vibrations nce tus nqi ntawm diffusion ntawm dej thiab tshuaj yaj nyob rau hauv nws los ntawm daim nyias nyias ntawm cov nroj tsuag hlwb, uas ua tiav ib tug ntau ceev cov nyhuv ntawm fungi thiab kab mob.

Ultrasound kuj muaj qhov tsis zoo rau tib neeg lub hlwb ntawm cov kab mob siab dua. Thaum irradiating cov qe ntshav liab (erythrocytes), cov hauv qab no tau pom: lawv poob lawv cov duab qub thiab ncab; Nyob rau tib lub sijhawm, lawv cov tsos mob tshwm sim (raws li qhov tshwm sim ntawm hemolysis). Thaum ntxiv irradiation, lawv thaum kawg ruptured thiab disintegrated mus rau hauv ntau nyias nyias.

Twb tau nyob rau xyoo 1928, nws tau tsim tias cov kab mob luminous raug rhuav tshem los ntawm ultrasound. Nyob rau hauv lub xyoo tom ntej, ib tug loj tus naj npawb ntawm tej hauj lwm tau luam tawm nyob rau hauv cov nyhuv ntawm ultrasonic nthwv dej ntawm cov kab mob thiab cov kab mob. Nyob rau tib lub sijhawm, nws tau pom tias cov txiaj ntsig tuaj yeem muaj ntau haiv neeg: ntawm ib sab, nce agglutination, poob ntawm virulence lossis tag nrho cov kab mob tuag tau pom, ntawm qhov tod tes, cov txiaj ntsig rov qab kuj tau sau tseg - nce hauv tus naj npawb ntawm cov neeg siv tau. Qhov kawg tshwm sim tshwj xeeb tshaj yog tom qab lub sij hawm luv luv irradiation thiab tuaj yeem piav qhia los ntawm qhov tseeb tias thaum lub sij hawm luv luv irradiation, ua ntej ntawm tag nrho cov, kev sib cais ntawm cov kab mob ntawm cov kab mob tshwm sim, vim hais tias txhua tus neeg ntawm tes ua rau ib tug tshiab colony.

Nws tau pom tias typhoid rods raug tua tag nrho los ntawm ultrasound nrog lub zaus ntawm 4, 6 MHz, thaum staphylococci thiab streptococci puas tsuas yog ib feem. Nrog rau kev tuag ntawm cov kab mob, lawv cov kev sib cais tshwm sim ib txhij, piv txwv li, kev puas tsuaj ntawm cov qauv morphological, yog li ntawd tom qab qhov kev txiav txim ntawm ultrasound, tsis tsuas yog tus naj npawb ntawm cov cheeb tsam hauv ib kab lis kev cai txo qis, tab sis suav tus naj npawb ntawm cov tib neeg qhia txog kev txo qis hauv cov kab mob. morphologically khaws cia cov ntaub ntawv ntawm cov kab mob. Thaum irradiated nrog ultrasound ntawm zaus ntawm 960 kHz, cov kab mob uas muaj qhov loj ntawm 20-75 µm raug rhuav tshem sai dua thiab ntau dua li cov kab mob uas muaj qhov loj ntawm 8-12 µm [23].

Ntawm Moscow Central Research Institute of Traumatology thiab Orthopedics muaj npe tom qab V. I. NN Priorov ua kev tshawb fawb [24] ntawm cov nyhuv ntawm qhov tsawg zaus ultrasonic cavitation ntawm cov haujlwm tseem ceeb ntawm ntau hom staphylococcus. Hauv kev sim hauv vitro, cov txiaj ntsig hauv qab no tau txais. Kev kho ultrasonic tau ua tiav ntawm qhov kub ntawm 32 ° C siv lub ultrasonic disintegrator los ntawm MSE (Great Britain), uas muaj cov txheej txheem hauv qab no: lub zog 150 W, vibration zaus 20 kHz, amplitude 55 μm. Lub sij hawm raug yog 1, 2, 5 "7, 10 feeb. Rau txhua qhov kis tau, cais vials nrog 5 ml ntawm cov kab mob tua kab mob uas muaj 2500 microbial lub cev hauv 1 ml ntawm cov kua siv ntawm qhov nruab nrab tam sim ntawd tom qab kev kho mob ultrasonic tsis tsuas yog tsis tau. tsis muaj zog, tab sis ntawm qee qhov kev tawm suab (1-3 min) nws txawm me ntsis intensifies. yog insignificant thiab yuav luag tsis txawv ntawm kev tswj. Cov nyhuv ntawm ultrasound ntawm cov kab mob tej zaum yuav tshwm sim ^ tsis tam sim ntawd, tab sis tom qab ib pliag, tsim nyog rau lub txoj kev loj hlob ntawm metabolic ntshawv siab nyob rau hauv lub hlwb, yog li ntawd, lub inoculation ntawm staphylococcus ntawm cov khoom noj khoom noj khoom haus tau kawm 24, 36, thiab 48 teev tom qab ultrasound Ua ntej sowing ntawm Petri tais diav, sonicated hom ntawm staphylococcus tau cultivated. thiab nyob rau hauv cov hlab ntsha nrog broth nyob rau hauv ib tug thermostat ntawm 37 ° C. Nws tau pom tias tom qab 24 thiab 36 teev tom qab kev kho mob ultrasonic, tus naj npawb ntawm cov kab mob loj hlob ntawm staphylococci piv nrog kev tswj qis, cov noob tus nqi ntawm staphylococcus yog inversely proportional rau lub sij hawm ntawm sounding ntawm cov kab mob. Tom qab 7-10 feeb ntawm sonication, lub noob yog tsis muaj kev loj hlob, los yog ib colonies tsis raug rau staphylococcus loj hlob ntawm Petri tais diav. Tom qab 48 teev, cov nyhuv inhibitory ntawm ultrasound tau tshaj tawm thiab pom nws tus kheej hauv kev txo qis hauv cov noob ntawm cov kab mob ntawm txhua qhov raug.

Kev tshawb fawb txog qhov rhiab heev ntawm cov kab mob uas muaj suab nrov rau kev ua ntawm qee cov tshuaj tua kab mob thiab tshuaj tua kab mob tau pom tias hauv 8 ntawm 13 cov tshuaj siv, qhov tsawg kawg nkaus inhibitory concentration tom qab kev kho ultrasonic ntawm staphylococcus poob 2-4 zaug. Qhov no qhia txog qhov ua tau ntawm kev siv ua ke ntawm cov zaus ultrasonic vibrations tsawg thiab cov tshuaj tua kab mob kom muaj txiaj ntsig zoo dua ntawm cov kab mob microbial [7, 10].

Qhov kev puas tsuaj ntawm ultrasonic tsis yog nyob ntawm qhov concentration ntawm cov kab mob raug tshem tawm. Nyob rau hauv ib tug tuab heev thiab, yog li ntawd, viscous suspension heev, tsis muaj kev puas tsuaj ntawm cov kab mob yog pom, tab sis tsuas yog cua sov yuav tsum tau muab sau tseg. Cov kab mob sib txawv ntawm tib hom kab mob tuaj yeem muaj tus cwj pwm txawv ntawm ultrasound irradiation [11].

Yog li, peb tuaj yeem txiav txim siab tias qhov cuam tshuam ntawm ultrasound ntawm biomaterial feem ntau thiab kab mob, tshwj xeeb, nyob ntawm ntau yam ib puag ncig thiab ntawm lub xeev ntawm cov teeb meem nyob, thiab qhov tseeb nws yog qhov nyuaj rau kwv yees.

Kev sim ntawm ultrasonic tu ntawm titanium intraosseous kho hniav implants nyob rau hauv ntau yam ua hauj lwm daws tau nqa tawm nyob rau hauv lub department ntawm SSTU.

Kev tu cov khoom yog qhov ua tau zoo dua, lawv nyob ze rau ntawm qhov chaw emitter. Nrog kev ncua deb ntawm lub emitter, qhov kev siv ntawm ultrasonic vibrations hloov raws li ib tug zoo tagnrho nkhaus. Qhov txiaj ntsig zoo tshaj plaws tau txais ntawm qhov kev siv ntawm 16 W / cm2 hauv kais dej thiab dej ua haujlwm ntawm 50 + 5 ° C nrog sulfanol concentration ntawm 0.25% nrog lub sijhawm sonication ntawm 5-10 feeb (Fig. 2.1). Cov khoom sonicated tau nyob ntawm qhov deb ntawm tsis ntau tshaj 10 hli los ntawm emitting nto.

Yog li, raws li cov kev sim, qhov nce ntawm qhov kev siv ntawm 0.4 mus rau 16 W / cm2 muab kev txhim kho ntawm kev tu kom zoo (Fig. 2.2), tab sis 100% sterilization ntawm cov khoom tsis ua tiav nyob rau hauv txhua hom.